Boyer M. auteur aut IRD Yutin N. auteur aut IRD Pagnier I. auteur aut IRD Barrassi L. auteur aut IRD Fournous G. auteur aut IRD Espinosa L. auteur aut IRD Robert C. auteur aut IRD Azza S. auteur aut IRD Sun S. Y. auteur aut IRD Rossmann M. G. auteur aut IRD Suzan-Monti M. auteur aut IRD La Scola B. auteur aut IRD Koonin E. V. auteur aut IRD Raoult Didier auteur aut IRD text journalArticle eng text/pdf born digital access Giant viruses such as Mimivirus isolated from amoeba found in aquatic habitats show biological sophistication comparable to that of simple cellular life forms and seem to evolve by similar mechanisms, including extensive gene duplication and horizontal gene transfer (HGT), possibly in part through a viral parasite, the virophage. We report here the isolation of "Marseille' virus, a previously uncharacterized giant virus of amoeba. The virions of Marseillevirus encompass a 368-kb genome, a minimum of 49 proteins, and some messenger RNAs. Phylogenetic analysis of core genes indicates that Marseillevirus is the prototype of a family of nucleocytoplasmic large DNA viruses (NCLDV) of eukaryotes. The genome repertoire of the virus is composed of typical NCLDV core genes and genes apparently obtained from eukaryotic hosts and their parasites or symbionts, both bacterial and viral. We propose that amoebae are "melting pots' of microbial evolution where diverse forms emerge, including giant viruses with complex gene repertoires of various origins. specialized giant virus horizontal gene transfer nucleocytoplasmic large DNA virus viral evolution 052 106 51 21848-21853 2009 0027-8424 https://www.documentation.ird.fr/hor/fdi:010048512 10.1073/pnas.0911354106 0027-8424 [F B010048512] https://www.documentation.ird.fr/hor/fdi:010048512 https://www.documentation.ird.fr/intranet/publi/2010/01/010048512.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2010-02-08 2017-08-23 fdi:010048512 fre