@article{fdi:010048508,
  title = {{H}igh prevalence of {M}ethanobrevibacter smithii and {M}ethanosphaera stadtmanae detected in the human gut using an improved {DNA} detection protocol},
  author = {{D}ridi, {B}. and {H}enry, {M}. and {E}l {K}hechine, {A}. and {R}aoult, {D}idier and {D}rancourt, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he low and variable prevalence of {M}ethanobrevibacter smithii and {M}ethanosphaera stadtmanae {DNA} in human stool contrasts with the paramount role of these methanogenic {A}rchaea in digestion processes. {W}e hypothesized that this contrast is a consequence of the inefficiencies of current protocols for archaeon {DNA} extraction. {W}e developed a new protocol for the extraction and {PCR}-based detection of {M}. smithii and {M}. stadtmanae {DNA} in human stool. {M}ethodology/{P}rincipal {F}indings: {S}tool specimens collected from 700 individuals were filtered, mechanically lysed twice, and incubated overnight with proteinase {K} prior to {DNA} extraction using a commercial {DNA} extraction kit. {T}otal {DNA} was used as a template for quantitative real-time {PCR} targeting {M}. smithii and {M}. stadtmanae 16{S} r{RNA} and rpo{B} genes. {A}mplification of 16{S} r{RNA} and rpo{B} yielded positive detection of {M}. smithii in 95.7% and {M}. stadtmanae in 29.4% of specimens. {S}equencing of 16{S} r{RNA} gene {PCR} products from 30 randomly selected specimens ( 15 for {M}. smithii and 15 for {M}. stadtmanae) yielded a sequence similarity of 99-100% using the reference {M}. smithii {ATCC} 35061 and {M}. stadtmanae {DSM} 3091 sequences. {C}onclusions/{S}ignificance: {I}n contrast to previous reports, these data indicate a high prevalence of the methanogens {M}. smithii and {M}. stadtmanae in the human gut, with the former being an almost ubiquitous inhabitant of the intestinal microbiome.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {4},
  numero = {9},
  pages = {e7063},
  ISSN = {1932-6203},
  year = {2009},
  DOI = {10.1371/journal.pone.0007063},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048508},
}