@article{fdi:010048494,
  title = {{S}ensitivity of {IFN}-gamma release assay to detect latent tuberculosis infection is retained in {HIV}-infected patients but dependent on {HIV}/{AIDS} progression},
  author = {{K}aram, {F}. and {M}bow, {F}. and {F}letcher, {H}. and {S}enghor, {C}. {S}. and {C}oulibaly, {K}. {D}. and {L}e{F}evre, {A}. {M}. and {G}ueye, {N}. {F}. {N}. and {D}ieye, {T}. and {S}ow, {P}. {S}. and {M}boup, {S}. and {L}ienhardt, {C}hristian},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground. {D}etection and treatment of latent {TB} infection ({LTBI}) in {HIV} infected individuals is strongly recommended to decrease morbidity and mortality in countries with high levels of {HIV}. {O}bjective. {T}o assess the validity of a newly developed in-house {ELISPOT} interferon-c release assay ({IGRA}) for the detection of {LTBI} amongst {HIV} infected individuals, in comparison with the {T}uberculin {S}kin {T}est ({TST}). {M}ethodology/{P}rincipal {F}indings. {ESAT}6/{CFP}10 ({EC}) {ELISPOT} assays were performed, together with a {TST}, in 285 {HIV} infected individuals recruited in {HIV} clinics in {D}akar, {S}enegal, who had no signs of active {TB} at time of enrolment. {T}hirty eight of the subjects (13.3%) failed to respond to {PHA} stimulation and were excluded from the analysis. {I}n the 247 remaining patients, response to {PHA} did not vary according to {CD}4 cell count categories (p = 0.51). {EC} {ELISPOT} was positive in 125 (50.6%) subjects, while 53 (21.5%) had a positive {TST}. {C}oncordance between {EC} {ELISPOT} and {TST} was observed in 151 patients (61.1%) (kappa = 0.23). {T}he proportion of subjects with a positive response to the {EC} {ELISPOT} assay decreased with declining {CD}4 counts (p trend = 0.001), but were consistently higher than the proportion of {TST} responders. {I}n multivariate analysis, the risk of being {EC}-{ELISPOT} positive in {HIV} infected individuals was associated with age, {CD}4 count and {HIV}-1 strain. {C}onclusion. {O}ur study indicates that {IGRA}s using {M}. tuberculosis specific antigens are likely to retain their validity for the diagnosis of {LTBI} among {HIV} positive individuals, but may be impaired by {T}-cell anergy in severely immuno-suppressed individuals.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {3},
  numero = {1},
  pages = {e1441},
  ISSN = {1932-6203},
  year = {2008},
  DOI = {10.1371/journal.pone.0001441},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048494},
}