@article{fdi:010048479,
  title = {{C}ryopreservation of in vitro grown shoot tips and apical meristems of the forage legume {A}rachis pintoi},
  author = {{R}ey, {H}. {Y}. and {F}aloci, {M}. and {M}edina, {R}. and {D}olce, {N}. and {M}royinski, {L}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{A} cryopreservation protocol using the encapsulation-dehydration procedure was established for shoot tips (2-3 mm in length) and meristems (0.3-0.5 mm) sampled from in vitro plantlets of diploid and triploid cytotypes of {A}rachis pintoi. {T}he optimal protocol was the following: after dissection, explants were precultured for 24 h on establishment medium ({EM}), encapsulated in calcium alginate beads and pretreated in liquid {EM} medium with daily increasing sucrose concentration (0.5, 0.75, 1.0 {M}) and desiccated to 22-23% moisture content (fresh weight basis). {E}xplants were frozen using slow cooling (1 degrees {C} min(-1) from 25 degrees {C} to -30 degrees {C} followed by direct immersion in liquid nitrogen), thawed rapidly and post-cultured in liquid {EM} medium enriched with daily decreasing sucrose concentrations (0.75, 0.50, 0.1 {M}). {E}xplants were then transferred to solid {EM} medium in order to achieve shoot regeneration, then on {M}urashige and {S}koog medium supplemented with 0.05 mu {M} naphthalene acetic acid to induce rooting of shoots. {W}ith this procedure, 53% and 56% of cryopreserved shoot tips of the diploid and triploid cytotypes, respectively, survived and formed plants. {H}owever, only 16% of cryopreserved meristems of both cytotypes regenerated plants. {U}sing ten isozyme systems and seven {RAPD} profiles, no modification induced by cryopreservation could be detected in plantlets regenerated from cryopreserved material.},
  keywords = {apical meristems ; cryopreservation ; encapsulation-dehydration ; genetic ; stability ; shoot tips},
  booktitle = {},
  journal = {{C}ryoletters},
  volume = {30},
  numero = {5},
  pages = {347--358},
  ISSN = {0143-2044},
  year = {2009},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048479},
}