Yakhlef S. B. auteur aut IRD Kerdouh B. auteur aut IRD Mousain D. auteur aut IRD Ducousso M. auteur aut IRD Duponnois Robin auteur aut IRD Abourouh M. auteur aut IRD text journalArticle eng text/pdf born digital access Interspecific variation among 87 sporocarps of fungi belonging to 15 genera and 39 species were evaluated by analyzing the internal transcribed spacer (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 500 and 950 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the species. Cleavage of amplified fragments with the restriction enzymes Alu I, EcoR I and Hinf I revealed extensive polymorphism. The fifteen genera and most species presented specific restriction patterns. The only species not identifiable by a specific pattern belonged to the genera Russula (R. decipiens and R. straminea). These species might be considered as closely related species. The Pisolithus sporocarps had two ITS-RFLP types with one dominating. ITS sequencing confirms that the two RFLP types correspond to two distinct species of Pisolithus. Our data show the potential of ITS region PCR-RFLP for the molecular characterization of ectomycorrhizal fungi and their identification and monitoring in artificial inoculation programs. specialized Diversity fungi Internal Transcribed Spacer (ITS) ribosomal DNA (rDNA) PCR-RFLP Morocco 074 13 4 521-528 2009 1370-6233 https://www.documentation.ird.fr/hor/fdi:010048478 1370-6233 [F B010048478] https://www.documentation.ird.fr/hor/fdi:010048478 https://www.documentation.ird.fr/intranet/publi/2010/01/010048478.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2010-02-08 2017-08-23 fdi:010048478 fre