@article{fdi:010048478,
  title = {{P}hylogenetic diversity of {M}oroccan cork oak woodlands fungi},
  author = {{Y}akhlef, {S}. {B}. and {K}erdouh, {B}. and {M}ousain, {D}. and {D}ucousso, {M}. and {D}uponnois, {R}obin and {A}bourouh, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}nterspecific variation among 87 sporocarps of fungi belonging to 15 genera and 39 species were evaluated by analyzing the internal transcribed spacer ({ITS}) of the r{DNA} region using restriction fragment length polymorphism ({RFLP}). {T}he {ITS} region was first amplified by polymerase chain reaction ({PCR}) with specific primers and then cleaved with different restriction enzymes. {A}mplification products, which ranged between 500 and 950 base pairs (bp), were obtained for all the isolates analyzed. {T}he degree of polymorphism observed did not allow proper identification of most of the species. {C}leavage of amplified fragments with the restriction enzymes {A}lu {I}, {E}co{R} {I} and {H}inf {I} revealed extensive polymorphism. {T}he fifteen genera and most species presented specific restriction patterns. {T}he only species not identifiable by a specific pattern belonged to the genera {R}ussula ({R}. decipiens and {R}. straminea). {T}hese species might be considered as closely related species. {T}he {P}isolithus sporocarps had two {ITS}-{RFLP} types with one dominating. {ITS} sequencing confirms that the two {RFLP} types correspond to two distinct species of {P}isolithus. {O}ur data show the potential of {ITS} region {PCR}-{RFLP} for the molecular characterization of ectomycorrhizal fungi and their identification and monitoring in artificial inoculation programs.},
  keywords = {{D}iversity ; fungi ; {I}nternal {T}ranscribed {S}pacer ({ITS}) ; ribosomal {DNA} ; (r{DNA}) ; {PCR}-{RFLP} ; {M}orocco},
  booktitle = {},
  journal = {{B}iotechnologie {A}gronomie {S}ociete et {E}nvironnement},
  volume = {13},
  numero = {4},
  pages = {521--528},
  ISSN = {1370-6233},
  year = {2009},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048478},
}