@article{fdi:010048441,
  title = {{O}ccurrence of fungi in combs of fungus-growing termites ({I}soptera: {T}ermitidae, {M}acrotermitinae)},
  author = {{G}uedegbe, {H}erbert and {M}iambi, {E}douard and {P}ando, {A}nne and {R}oman, {J}ocelyne and {H}oungnandan, {P}. and {R}ouland {L}ef{\`e}vre, {C}orinne},
  editor = {},
  language = {{ENG}},
  abstract = {{F}ungus-growing termites cultivate their mutualistic basidiomycete {T}ermitomyces species on a substrate called a fungal comb. {H}ere, the {S}uicide {P}olymerase {E}ndonuclease {R}estriction ({S}u{PER}) method was adapted for the first time to a fungal study to determine the entire fungal community of fungal combs and to test whether fungi other than the symbiotic cultivar interact with termite hosts. {O}ur molecular analyses show that although active combs are dominated by {T}ermitomyces fungi isolated with direct {P}olymerase {E}ndonuclease {R}estriction - {D}enaturing {G}radient {G}el {E}lectrophoresis ({PCR}-{DGGE}), they can also harbor some filamentous fungi and yeasts only revealed by {S}u{PER} {PCR}-{DGGE}. {T}his is the first molecular evidence of the presence of non-{T}ermitomyces species in active combs. {H}owever, because there is no evidence for a species-specific relationship between these fungi and termites, they are mere transient guests with no specialization in the symbiosis. {I}t is however surprising to notice that termite-associated {X}ylaria strains were not isolated from active combs even though they are frequently retrieved when nests are abandoned by termites. {T}his finding highlights the implication of fungus-growing termites in the regulation of fungi occurring within the combs and also suggests that they might not have any particular evolutionary-based association with {X}ylaria species.},
  keywords = {{F}ungal combs ; {F}ungal communities ; {ITS} ; {M}onocultures ; {S}u{PER} method ; {T}ermite-associated fungi},
  booktitle = {},
  journal = {{M}ycological {R}esearch},
  volume = {113},
  numero = {{P}art 10},
  pages = {1039--1045},
  ISSN = {0953-7562},
  year = {2009},
  DOI = {10.1016/j.mycres.2009.06.008},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048441},
}