@article{fdi:010048417,
  title = {{E}xploring the molecular basis of insecticide resistance in the dengue vector {A}edes aegypti : a case study in {M}artinique {I}sland ({F}rench {W}est {I}ndies)},
  author = {{M}arcombe, {S}{\'e}bastien and {P}oupardin, {R}. and {D}arriet, {F}r{\'e}d{\'e}ric and {R}eynaud, {S}. and {B}onnet, {J}ulien and {S}trode, {C}. and {B}rengues, {C}{\'e}cile and {Y}ebakima, {A}. and {R}anson, {H}. and {C}orbel, {V}incent and {D}avid, {J}. {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he yellow fever mosquito {A}edes aegypti is a major vector of dengue and hemorrhagic fevers, causing up to 100 million dengue infections every year. {A}s there is still no medicine and efficient vaccine available, vector control largely based on insecticide treatments remains the only method to reduce dengue virus transmission. {U}nfortunately, vector control programs are facing operational challenges with mosquitoes becoming resistant to commonly used insecticides. {R}esistance of {A}e. aegypti to chemical insecticides has been reported worldwide and the underlying molecular mechanisms, including the identification of enzymes involved in insecticide detoxification are not completely understood. {R}esults: {T}he present paper investigates the molecular basis of insecticide resistance in a population of {A}e. aegypti collected in {M}artinique ({F}rench {W}est {I}ndies). {B}ioassays with insecticides on adults and larvae revealed high levels of resistance to organophosphate and pyrethroid insecticides. {M}olecular screening for common insecticide target-site mutations showed a high frequency (71%) of the sodium channel 'knock down resistance' (kdr) mutation. {E}xposing mosquitoes to detoxification enzymes inhibitors prior to bioassays induced a significant increased susceptibility of mosquitoes to insecticides, revealing the presence of metabolic-based resistance mechanisms. {T}his trend was biochemically confirmed by significant elevated activities of cytochrome {P}450 monooxygenases, glutathione {S}-transferases and carboxylesterases at both larval and adult stages. {U}tilization of the microarray {A}edes {D}etox {C}hip containing probes for all members of detoxification and other insecticide resistance-related enzymes revealed the significant constitutive over-transcription of multiple detoxification genes at both larval and adult stages. {T}he over-transcription of detoxification genes in the resistant strain was confirmed by using real-time quantitative {RT}-{PCR}. {C}onclusion: {T}hese results suggest that the high level of insecticide resistance found in {A}e. aegypti mosquitoes from {M}artinique island is the consequence of both target-site and metabolic based resistance mechanisms. {I}nsecticide resistance levels and associated mechanisms are discussed in relation with the environmental context of {M}artinique {I}sland. {T}hese finding have important implications for dengue vector control in {M}artinique and emphasizes the need to develop new tools and strategies for maintaining an effective control of {A}edes mosquito populations worldwide.},
  keywords = {},
  booktitle = {},
  journal = {{B}mc {G}enomics},
  volume = {10},
  numero = {},
  pages = {494},
  ISSN = {1471-2164},
  year = {2009},
  DOI = {10.1186/1471-2164-10-494},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048417},
}