@article{fdi:010048374,
  title = {{R}eal-time {PCR} strategy and detection of bacterial agents of lymphadenitis},
  author = {{A}ngelakis, {E}. and {R}oux, {V}. and {R}aoult, {D}idier and {R}olain, {J}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he aim of this study was to compare 16 {S} r{RNA} gene amplification and sequencing with a systematic real-time {PCR} assay screening strategy that includes all common known pathogens recovered from lymph node biopsy specimens. {L}ymph node biopsy samples sent to our laboratory from {J}anuary 2007 to {D}ecember 2008 were tested in the study. {L}ymph nodes were screened for the presence of any bacteria by {PCR} amplification and sequencing targeting the 16 {S} r{RNA} gene and also by a specific real-time {PCR} strategy that includes {B}artonella henselae, mycobacteria, {F}rancisella tularensis, and {T}ropheryma whipplei. {B}y testing 491 lymph nodes, we found that the sensitivity of our specific real-time {PCR} assay strategy was significantly higher than 16 {S} r{RNA} {PCR} amplification and sequencing for the detection of {B}artonella henselae (142 vs 98; p < 10(-4)), {F}rancisella tularensis (16 vs 10, p < 10(-4)), and mycobacteria (8 versus 3, p < 10(-4)). {N}one of the samples was positive for {T}ropheryma whipplei. {O}ur study demonstrates the usefulness and specificity of a systematic real-time {PCR} strategy for molecular analysis of lymph node biopsy specimens and the higher sensitivity compared with standard 16 {S} r{RNA} gene amplification and sequencing.},
  keywords = {},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {C}linical {M}icrobiology and {I}nfectious {D}iseases},
  volume = {28},
  numero = {11},
  pages = {1363--1368},
  ISSN = {0934-9723},
  year = {2009},
  DOI = {10.1007/s10096-009-0793-6},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048374},
}