@article{fdi:010048323,
  title = {{V}igna mungo, {V}. radiata and {V}. unguiculata plants sampled in different agronomical-ecological-climatic regions of {I}ndia are nodulated by {B}radyrhizobium yuanmingense},
  author = {{A}ppunu, {C}. and {N}'{Z}ou{\'e}, {A}ng{\`e}le and {M}oulin, {L}ionel and {D}epret, {G}. and {L}aguerre, {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{V}igna mungo, {V}igna radiata and {V}igna unguiculata are important legume crops cultivated in {I}ndia, but little is known about the genetic resources in native rhizobia that nodulate these species. {T}o identify these bacteria, a core collection of 76 slow-growing isolates was built from root nodules of {V} mungo, {V} radiata and {V} unguiculata plants grown at different sites within three agro-ecological-climatic regions of {I}ndia. {T}he genetic diversity of the bacterial collection was assessed by restriction fragment length polymorphism ({RFLP}) analysis of {PCR}-amplified {DNA} fragments of the 16{S}-23{S} r{DNA} intergenic spacer ({IGS}) region, and the symbiotic genes nif{H} and nod{C}. {O}ne r{DNA} {IGS} type grouped 91% of isolates, but more diversity was found at the symbiotic loci (17 symbiotic genotypes). {O}verall, no host plant specificity was shown, the three host plant species sharing common bradyrhizobial genotypes that represented 62% of the collection. {S}imilarly, the predominant genotypes were found at most sampling sites and in all agro-ecological-climatic regions. {P}hylogenies inferred from {IGS} sequencing and multi-locus sequence analysis of the dna{K}, gln{II} and rec{A} genes indicated that all isolates but one were clustered with the {B}radyrhizobium yuanmingense species. {T}he nif{H} phylogeny also grouped the different nif haplotypes within a cluster including {B}. yuanmingense, except for one infrequent nif haplotype which formed a new lineage within the {B}radyrhizobium genus. {T}hese results may reflect a long history of co-evolution between {B}. yuanmingense and {V}igna spp. in {I}ndia, while intra-species polymorphism detected in the symbiotic loci may be linked with the long history of diversification of {B}. yuanmingense coinciding with that of its host legumes.},
  keywords = {{B}radyrhizobium ; {V}igna spp. ; {D}iversity ; {P}hylogeny ; {M}ulti-locus sequence analysis ; {S}ymbiotic genes},
  booktitle = {},
  journal = {{S}ystematic and {A}pplied {M}icrobiology},
  volume = {32},
  numero = {7},
  pages = {460--470},
  ISSN = {0723-2020},
  year = {2009},
  DOI = {10.1016/j.syapm.2009.05.005},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048323},
}