@article{fdi:010048264,
  title = {{B}iphasic haustorial differentiation of coffee rust ({H}emileia vastatrix race {II}) associated with defence responses in resistant and susceptible coffee cultivars},
  author = {{R}amiro, {D}aniel and {E}scoute, {J}. and {P}etitot, {A}nne-{S}ophie and {N}icole, {M}ichel and {M}aluf, {M}. {P}. and {F}ernandez, {D}iana},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he objective of this {S}tudy was to assess whether defence responses in coffee ({C}offea arabica) were linked to a specific developmental stage of the rust fungus {H}emileia vastatrix. {H}istological observations in compatible and incompatible high-yielding {B}razilian {C}offee cultivars; showed that the fungus produced 'pioneer' haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed 'secondary haustoria' which invade mesophyll cells. {I}n the incompatible interaction between {R}ace {II} and cv. {T}upi, a strong and transient {H}2{O}2 generation at infection sites was detected at 39 h {P}ost inoculation (h{P}i) during secondary haustoria formation. {I}n addition, clear-cut differences in defence gene expression between compatible and incompatible interactions were only observed during the secondary haustoria formation. {T}ranscripts of the pathogenesis-related ({PR}) genes {C}a{PR}1b and {C}a{PR}10 accumulated to maximal levels at 39 hpi (38- and 86-fold, respectively) in the incompatible interaction, but stayed at low levels in the compatible interaction. {I}n contrast, the {C}a{WRKY}1 gene and the {C}a{RLK} gene were only induced in the {S}usceptible cultivar. {T}hese results indicated that the specific resistance of cv. {T}upi {W}as expressed after differentiation of the {H}. vastatrix secondary haustoria. {A}nalysis showed no evidence of specific recognition {O}f {C}offee rust at the pioneer haustoria stage, {S}uggesting that haustoria components are not recognized by, or not secreted into, the {S}ubsidiary and adjacent cells of the stomata. {A}dditionally, the present {S}tudy provides new insights into the colonization process of the coffee rust fungus.},
  keywords = {{C}offea arabica ; pathogenesis-related genes ; quantitative real-time {PCR} ; reactive oxygen species},
  booktitle = {},
  journal = {{P}lant {P}athology},
  volume = {58},
  numero = {5},
  pages = {944--955},
  ISSN = {0032-0862},
  year = {2009},
  DOI = {10.1111/j.1365-3059.2009.02122.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048264},
}