Armougom F. auteur aut IRD Bittar F. auteur aut IRD Stremler N. auteur aut IRD Rolain J. M. auteur aut IRD Robert C. auteur aut IRD Dubus J. C. auteur aut IRD Sarles J. auteur aut IRD Raoult Didier auteur aut IRD La Scola B. auteur aut IRD text journalArticle eng text/pdf born digital access Recent studies using 16S rRNA gene amplification followed by clonal Sanger sequencing in cystic fibrosis demonstrated that cultured microorganisms are only part of the infecting flora. The purpose of this paper was to compare pyrosequencing and clonal Sanger sequencing on sputum. The sputum of a patient with cystic fibrosis was analysed by culture, Sanger clone sequencing and pyrosequencing after 16S rRNA gene amplification. A total of 4,499 sequencing reads were obtained, which could be attributed to six consensus sequences, but the length of reads leads to fastidious data analysis. Compared to clonal Sanger sequencing and to cultivation results, pyrosequencing recovers greater species richness and gives a more reliable estimate of the relative abundance of bacterial species. The 16S pyrosequencing approach expands our knowledge of the microbial diversity of cystic fibrosis sputum. The current lack of phylogenetic resolution at the species level for the GS 20 sequencing reads will be overcome with the next generation of pyrosequencing apparatus. specialized 052 28 9 1151-1154 2009 0934-9723 https://www.documentation.ird.fr/hor/fdi:010048261 10.1007/s10096-009-0749-x 0934-9723 [F B010048261] https://www.documentation.ird.fr/hor/fdi:010048261 https://www.documentation.ird.fr/intranet/publi/2009/10/010048261.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2009-11-09 2017-08-23 fdi:010048261 fre