@article{fdi:010048261,
  title = {{M}icrobial diversity in the sputum of a cystic fibrosis patient studied with 16{S} r{DNA} pyrosequencing},
  author = {{A}rmougom, {F}. and {B}ittar, {F}. and {S}tremler, {N}. and {R}olain, {J}. {M}. and {R}obert, {C}. and {D}ubus, {J}. {C}. and {S}arles, {J}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ecent studies using 16{S} r{RNA} gene amplification followed by clonal {S}anger sequencing in cystic fibrosis demonstrated that cultured microorganisms are only part of the infecting flora. {T}he purpose of this paper was to compare pyrosequencing and clonal {S}anger sequencing on sputum. {T}he sputum of a patient with cystic fibrosis was analysed by culture, {S}anger clone sequencing and pyrosequencing after 16{S} r{RNA} gene amplification. {A} total of 4,499 sequencing reads were obtained, which could be attributed to six consensus sequences, but the length of reads leads to fastidious data analysis. {C}ompared to clonal {S}anger sequencing and to cultivation results, pyrosequencing recovers greater species richness and gives a more reliable estimate of the relative abundance of bacterial species. {T}he 16{S} pyrosequencing approach expands our knowledge of the microbial diversity of cystic fibrosis sputum. {T}he current lack of phylogenetic resolution at the species level for the {GS} 20 sequencing reads will be overcome with the next generation of pyrosequencing apparatus.},
  keywords = {},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {C}linical {M}icrobiology and {I}nfectious {D}iseases},
  volume = {28},
  numero = {9},
  pages = {1151--1154},
  ISSN = {0934-9723},
  year = {2009},
  DOI = {10.1007/s10096-009-0749-x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048261},
}