Acquisition of callogenic capacity in date palm leaf tissues in response to 2,4-D treatment

Acquisition of callogenic capacity in date palm leaf tissues in response to 2,4-D treatment Gueye B. auteur aut IRD Morcillo F. auteur aut IRD Collin Myriam auteur aut IRD Gargani D. auteur aut IRD Overvoorde P. auteur aut IRD Aberlenc Bertossi Frédérique auteur aut IRD Tranbarger Timothy auteur aut IRD Sane D. auteur aut IRD Tregear James auteur aut IRD Borgel Alain auteur aut IRD Verdeil J. L. auteur aut IRD text journalArticle eng text/pdf born digital access While callogenesis in date palm can be initiated by culturing immature leaf segments on medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), it is difficult to obtain callus from certain genotypes. To better understand the mechanisms of callogenesis in date palm, we studied the developmental events underlying this process at the cellular level. The callogenic capacity of leaf segments depends on the state of cell differentiation. The most callogenic segments were within the leaf elongation zone, required polar auxin transport to initiate callogenesis and contained the highest quantities of free endogenous indole-3-acetic acid. At the cellular level, callus induction involves two spatially and temporally separated events. The first event involves cells from the fascicular parenchyma in an equatorial plane perpendicular to the vascular axis that within 2 days of culture in the presence of 2,4-D, acquire structural and ultrastructural features typically observed in meristematic cells. The second event occurs 3 days later and is characterised by the modification of adjacent perivascular sheath cells. The latter cells become callogenic in that they reinitiated their cell cycles and undergo cell division leading to callus formation. These data provide evidence that callus initiation in leaf vascular tissue of date palm involves a sequential response of two distinct cell types to auxin and requires polar auxin transport. specialized Callogenic competence Cell reactivation Auxin transport Somatic embryogenesis Phoenix dactylifera 076 Plant Cell Tissue and Organ Culture 99 1 35-45 2009 0167-6857 https://www.documentation.ird.fr/hor/fdi:010048199 10.1007/s11240-009-9573-3 0167-6857 [F B010048199] https://www.documentation.ird.fr/hor/fdi:010048199 https://www.documentation.ird.fr/intranet/publi/2009/10/010048199.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2009-11-09 2023-06-14 fdi:010048199 fre