@article{fdi:010048199,
  title = {{A}cquisition of callogenic capacity in date palm leaf tissues in response to 2,4-{D} treatment},
  author = {{G}ueye, {B}. and {M}orcillo, {F}. and {C}ollin, {M}yriam and {G}argani, {D}. and {O}vervoorde, {P}. and {A}berlenc {B}ertossi, {F}r{\'e}d{\'e}rique and {T}ranbarger, {T}imothy and {S}ane, {D}. and {T}regear, {J}ames and {B}orgel, {A}lain and {V}erdeil, {J}. {L}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}hile callogenesis in date palm can be initiated by culturing immature leaf segments on medium containing 2,4-dichlorophenoxyacetic acid (2,4-{D}), it is difficult to obtain callus from certain genotypes. {T}o better understand the mechanisms of callogenesis in date palm, we studied the developmental events underlying this process at the cellular level. {T}he callogenic capacity of leaf segments depends on the state of cell differentiation. {T}he most callogenic segments were within the leaf elongation zone, required polar auxin transport to initiate callogenesis and contained the highest quantities of free endogenous indole-3-acetic acid. {A}t the cellular level, callus induction involves two spatially and temporally separated events. {T}he first event involves cells from the fascicular parenchyma in an equatorial plane perpendicular to the vascular axis that within 2 days of culture in the presence of 2,4-{D}, acquire structural and ultrastructural features typically observed in meristematic cells. {T}he second event occurs 3 days later and is characterised by the modification of adjacent perivascular sheath cells. {T}he latter cells become callogenic in that they reinitiated their cell cycles and undergo cell division leading to callus formation. {T}hese data provide evidence that callus initiation in leaf vascular tissue of date palm involves a sequential response of two distinct cell types to auxin and requires polar auxin transport.},
  keywords = {{C}allogenic competence ; {C}ell reactivation ; {A}uxin transport ; {S}omatic ; embryogenesis ; {P}hoenix dactylifera},
  booktitle = {},
  journal = {{P}lant {C}ell {T}issue and {O}rgan {C}ulture},
  volume = {99},
  numero = {1},
  pages = {35--45},
  ISSN = {0167-6857},
  year = {2009},
  DOI = {10.1007/s11240-009-9573-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048199},
}