Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Plant Cell Tissue and Organ Culture 73-81 Callogenesis Regeneration Somatic embryogenesis Palm Arecaceae 2009 fdi:010048198 ENG Plant Cell Tissue and Organ Culture 0167-6857 ISI:000269881000009 1 10.1007/s11240-009-9577-z https://www.documentation.ird.fr/hor/fdi:010048198 https://www.documentation.ird.fr/intranet/publi/2009/10/010048198.pdf 99 Horizon (IRD) Coconut is a cross pollinating palm, propagated only by seeds. Tissue culture is the only vegetative propagation method available for coconut. Consistent callogenesis was obtained by culturing unfertilised ovaries at -4 stage in CRI 72 medium containing 100 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1% activated charcoal. Callusing was improved by application of 9 mu M thidiazuron (TDZ). Embryogenic calli were subcultured onto somatic embryogenesis induction medium containing 66 mu M 2,4-D. Stunted growth was observed in the somatic embryos after subculture onto CRI 72 medium containing abscisic acid (ABA). Maturation of somatic embryos could be achieved in Y-3 medium without growth regulators. Conversion of somatic embryos was induced by adding gibberellic acid (GA(3)) to conversion medium containing 5 mu M 6-benzyladenine (BA) while 2-isopentyl adenine (2iP) increased the frequency of plant regeneration. A total of 83 plantlets was produced from 32 cultured ovaries. 076 UR142 Sri Lanka