%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Perera, P. I. P.
%A Vidhanaarachchi, V. R. M.
%A Gunathilake, T. R.
%A Yakandawala, D. M. D.
%A Hocher, Valérie
%A Verdeil, J. L.
%A Weerakoon, L. K.
%T Effect of plant growth regulators on ovary culture of coconut (Cocos nucifera L.)
%D 2009
%L fdi:010048198
%G ENG
%J Plant Cell Tissue and Organ Culture
%@ 0167-6857
%K Callogenesis ; Regeneration ; Somatic embryogenesis ; Palm ; Arecaceae
%M ISI:000269881000009
%N 1
%P 73-81
%R 10.1007/s11240-009-9577-z
%U https://www.documentation.ird.fr/hor/fdi:010048198
%> https://www.documentation.ird.fr/intranet/publi/2009/10/010048198.pdf
%V 99
%W Horizon (IRD)
%X Coconut is a cross pollinating palm, propagated only by seeds. Tissue culture is the only vegetative propagation method available for coconut. Consistent callogenesis was obtained by culturing unfertilised ovaries at -4 stage in CRI 72 medium containing 100 mu M 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1% activated charcoal. Callusing was improved by application of 9 mu M thidiazuron (TDZ). Embryogenic calli were subcultured onto somatic embryogenesis induction medium containing 66 mu M 2,4-D. Stunted growth was observed in the somatic embryos after subculture onto CRI 72 medium containing abscisic acid (ABA). Maturation of somatic embryos could be achieved in Y-3 medium without growth regulators. Conversion of somatic embryos was induced by adding gibberellic acid (GA(3)) to conversion medium containing 5 mu M 6-benzyladenine (BA) while 2-isopentyl adenine (2iP) increased the frequency of plant regeneration. A total of 83 plantlets was produced from 32 cultured ovaries.
%$ 076