@article{fdi:010048198,
  title = {{E}ffect of plant growth regulators on ovary culture of coconut ({C}ocos nucifera {L}.)},
  author = {{P}erera, {P}. {I}. {P}. and {V}idhanaarachchi, {V}. {R}. {M}. and {G}unathilake, {T}. {R}. and {Y}akandawala, {D}. {M}. {D}. and {H}ocher, {V}al{\'e}rie and {V}erdeil, {J}. {L}. and {W}eerakoon, {L}. {K}.},
  editor = {},
  language = {{ENG}},
  abstract = {{C}oconut is a cross pollinating palm, propagated only by seeds. {T}issue culture is the only vegetative propagation method available for coconut. {C}onsistent callogenesis was obtained by culturing unfertilised ovaries at -4 stage in {CRI} 72 medium containing 100 mu {M} 2,4-dichlorophenoxyacetic acid (2,4-{D}) and 0.1% activated charcoal. {C}allusing was improved by application of 9 mu {M} thidiazuron ({TDZ}). {E}mbryogenic calli were subcultured onto somatic embryogenesis induction medium containing 66 mu {M} 2,4-{D}. {S}tunted growth was observed in the somatic embryos after subculture onto {CRI} 72 medium containing abscisic acid ({ABA}). {M}aturation of somatic embryos could be achieved in {Y}-3 medium without growth regulators. {C}onversion of somatic embryos was induced by adding gibberellic acid ({GA}(3)) to conversion medium containing 5 mu {M} 6-benzyladenine ({BA}) while 2-isopentyl adenine (2i{P}) increased the frequency of plant regeneration. {A} total of 83 plantlets was produced from 32 cultured ovaries.},
  keywords = {{C}allogenesis ; {R}egeneration ; {S}omatic embryogenesis ; {P}alm ; {A}recaceae},
  booktitle = {},
  journal = {{P}lant {C}ell {T}issue and {O}rgan {C}ulture},
  volume = {99},
  numero = {1},
  pages = {73--81},
  ISSN = {0167-6857},
  year = {2009},
  DOI = {10.1007/s11240-009-9577-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048198},
}