@article{fdi:010048195,
  title = {{M}orphological and molecular analyses in {S}cleroderma species associated with some {C}aesalpinioid legumes, {D}ipterocarpaceae and {P}hyllanthaceae trees in southern {B}urkina {F}aso},
  author = {{S}anon, {K}. {B}. and {B}a, {A}. {M}. and {D}elaruelle, {C}. and {D}uponnois, {R}obin and {M}artin, {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A} combination of morphotypes, polymerase chain reaction/restriction fragment length polymorphism ({PCR}/{RFLP}) analyses and internal transcribed spacer ({ITS}) sequencing was used to investigate {S}cleroderma species that were collected from woodlands in {B}urkina {F}aso. {W}e harvested 52 specimens from 20 sites during rainy seasons between 1997 and 2000. {A}ccording to their morphological features, these specimens were initially characterised, and we then identified six species of {S}cleroderma. {T}wo of the species were clearly determined as {S}cleroderma dictyosporum {P}at. and {S}. verrucosum {P}ers. {T}he four remaining species were characteristically described as (1) displaying big spores with spines up to 2 {A} mu m ({S}cleroderma sp1), (2) producing spores without ornamentation ({S}cleroderma sp2), (3) spores with very small spines ({S}cleroderma sp3) and (4) with yellow sporocarps and sub-spherical spores ({S}cleroderma sp4). {T}he specimens were then analysed using {PCR}/{RFLP} of the intergenic regions of r{DNA}, {ITS} and {IGS}1 and {ITS} sequencing. {T}he restriction fragments obtained with two endonucleases, {H}inf{I} and {M}bo{I} on {ITS} and {IGS}1 regions, showed that some isolates of {S}. dictyosporum had the same patterns as isolates and basidiocarps of {S}cleroderma sp4 ({IR}265, {IR}408, {SP}4-2903). {I}solates of {S}cleroderma sp3 ({IR}252) had common restriction fragments as isolates of {S}. verrucosum ({IR}500, {IR}600). {I}ntraspecific differences were observed in the two previously determined species, as well as in {S}cleroderma sp2. {T}he {ITS} sequencing and phylogenetic analyses showed that the ribotypes identified by {PCR}/{RFLP} within these species might be phylogenetic species. {C}ombining these molecular results allowed regrouping the six morphological species in three sets of cryptic species: a first set with two species including {S}. dictyosporum {P}at., a second set with four species, including both {S}. verrucosum {P}ers. and {S}cleroderma sp1 and a third set with two species, including {S}cleroderma sp2. {T}hese investigations and the combined morphological and molecular analyses used to sort out species paved the way for identifying larger populations of {S}cleroderma species in {B}urkina {F}aso and other tropical zones.},
  keywords = {{S}cleroderma ; {M}orphology ; {I}dentification ; {PCR}/{RFLP} ; {ITS} sequencing ; {B}urkina {F}aso},
  booktitle = {},
  journal = {{M}ycorrhiza},
  volume = {19},
  numero = {8},
  pages = {571--584},
  ISSN = {0940-6360},
  year = {2009},
  DOI = {10.1007/s00572-009-0272-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010048195},
}