@article{fdi:010047687,
  title = {{I}ntrinsic disorder in viral proteins genome-linked : experimental and predictive analyses},
  author = {{H}{\'e}brard, {E}ug{\'e}nie and {B}essin, {Y}. and {M}ichon, {T}. and {L}onghi, {S}. and {U}versky, {V}.{N}. and {D}elalande, {F}. and {V}an {D}orsselaer, {A}. and {R}omero, {P}. and {W}alter, {J}. and {D}eclerck, {N}. and {F}argette, {D}enis},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {VP}gs are viral proteins linked to the 5' end of some viral genomes. {I}nteractions between several {VP}gs and eukaryotic translation initiation factors e{IF}4{E}s are critical for plant infection. {H}owever, {VP}gs are not restricted to phytoviruses, being also involved in genome replication and protein translation of several animal viruses. {T}o date, structural data are still limited to small picornaviral {VP}gs. {R}ecently three phytoviral {VP}gs were shown to be natively unfolded proteins. {R}esults: {I}n this paper, we report the bacterial expression, purification and biochemical characterization of two phytoviral {VP}gs, namely the {VP}gs of {R}ice yellow mottle virus ( {RYMV}, genus {S}obemovirus) and {L}ettuce mosaic virus ( {LMV}, genus {P}otyvirus). {U}sing far-{UV} circular dichroism and size exclusion chromatography, we show that {RYMV} and {LMV} {VP}gs are predominantly or partly unstructured in solution, respectively. {U}sing several disorder predictors, we show that both proteins are predicted to possess disordered regions. {W}e next extend theses results to 14 {VP}gs representative of the viral diversity. {D}isordered regions were predicted in all {VP}g sequences whatever the genus and the family. {C}onclusion: {B}ased on these results, we propose that intrinsic disorder is a common feature of {VP}gs. {T}he functional role of intrinsic disorder is discussed in light of the biological roles of {VP}gs.},
  keywords = {{PATHOLOGIE} {VEGETALE} ; {MALADIE} {DES} {PLANTES} ; {VIRUS} ; {PROTEINE} ; {INFECTION} ; {VARIABILITE} {GENETIQUE} ; {GENE}},
  booktitle = {},
  journal = {{V}irology {J}ournal},
  volume = {6},
  numero = {23},
  pages = {1--34},
  ISSN = {1743-422{X}},
  year = {2009},
  DOI = {10.1186/1743-422{X}-6-23},
  URL = {https://www.documentation.ird.fr/hor/fdi:010047687},
}