@article{fdi:010046770,
  title = {{R}eliability of {PCR}-based techniques for detection and discrimination of plant-parasitic nematodes of sugarcane},
  author = {{B}erry, {S}. and {F}argette, {M}ireille and {M}orand, {S}erge and {C}adet, {P}atrice},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n order to develop diagnostic tools for plant-parasitic nematodes of sugarcane in {S}outh {A}frica, this work investigated the potential biodiversity of these nematodes in the amount of nucleic acid available, the discrimination of species by a {PCR}-based method (targeting the {ITS} {I} region) and the effect of some experimental conditions on their detection or discrimination. {T}he {ITS} {I} amplified fragment varied in size, ranging from 400 bp ({M}eloidogyne javanica) to 1200 bp ({L}ongidorus pisi, {P}aratrichodorus minor, {X}iphinema elongatum, {X}iphinema {Z}ulu). {S}ome species discrimination was possible by discernible differences in {ITS} {I} amplified fragment sizes. {T}he potential. as well as shortcomings (inhibitions. competition) of this approach are assessed and further steps/outcomes discussed. {P}art of the work also focused on {DNA} extraction. {N}ematode genomic nucleic acid was extracted front five nematode species({P}rati,lenchus zeae, {H}elicotylenchus dihystera, {M}eloidogyne javanica. {X}iphinema elongatum and {P}aratrichodorus lobatus) and we discuss the significant differences obtained between species in the amount of nucleic acid extracted. {T}here were also marked differences in the quantity and quality of nucleic acid extracted from nematode {S}uspensions originating from soils, depending on the extraction buffer used.},
  keywords = {{DNA} extraction ; {DNA} quantification ; {H}elicotylenchus dihystera ; {ITS}1 ; {M}eloidogyne javanica ; {P}aratrichodorus lobatus ; {P}aratrichodorus minor ; {PCR} ; {P}ratylenchus zeae ; {X}iphinema elongatum},
  booktitle = {},
  journal = {{N}ematology},
  volume = {9},
  numero = {4},
  pages = {499--514},
  ISSN = {1388-5545},
  year = {2007},
  DOI = {10.1163/156854107781487297},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046770},
}