@article{fdi:010046340,
  title = {{F}requent transmission of hepatitis {E} virus among piglets in farms in {S}outhern {F}rance},
  author = {{K}aba, {M}. and {D}avoust, {B}. and {M}arie, {J}. {L}. and {B}arthet, {M}. and {H}enry, {M}. and {T}amalet, {C}. and {R}aoult, {D}idier and {C}olson, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he present study aimed to assess whether hepatitis {E} virus ({HEV}) is present in domestic pigs in {S}outhern {F}rance, and to determine the relationship between {HEV} sequences detected from pigs and from humans. {T}wo hundred fifteen sera, 207 stools, and 107 bile samples were collected from 3- or 6-month-old pigs from different regions of {S}outhern {F}rance. {P}ig {I}g{G} anti-{HEV} antibodies testing was performed using a commercial {ELISA} kit with minor modifications. {P}ig {HEV} {RNA} was tested by real-time {PCR} and sequencing assays using "in-house" protocols. {F}orty percent of pigs were {HEV}-seropositive. {S}ixty-five percent of 3-month-old pigs and none of 6-month-old pigs were {HEV} {RNA}-positive. {HEV} {RNA} was significantly more frequently detected from stools than from sera (65% vs. 22%; {P}<0.001). {P}hylogenetic analysis showed that pig {HEV} sequences belonged to genotype 3 and formed two clusters of genotype 3f and 3e. {N}ucleotide homology between pig {HEV} sequences of each cluster was high (>97%), and clusters were correlated with the geographical origin of pigs and with their repartition into pens and buildings in the pig farm. {B}ased on analysis of 331 nucleoticles, pig {HEV} sequences were close genetically to {HEV} sequences found from humans or pigs in {E}urope, and one showed complete nucleotide identity with an {HEV} sequence obtained in {F}rance from a human. {T}he present data indicate that 3-month-old pigs from {S}outhern {F}rance might represent a potential source of {HEV} transmission to humans, and stress the potential of {HEV} to cause epizootic infections in population of farm pigs. {J}. {M}ed. {V}irol. 81:1750-1759, 2009.},
  keywords = {hepatitis {E} virus ; pigs ; zoonosis ; epizootic transmission ; autochthonous transmission ; {F}rance},
  booktitle = {},
  journal = {{J}ournal of {M}edical {V}irology},
  volume = {81},
  numero = {10},
  pages = {1750--1759},
  ISSN = {0146-6615},
  year = {2009},
  DOI = {10.1002/jmv.21553},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046340},
}