@article{fdi:010046322,
  title = {{I}ncrimination of {E}ratyrus cuspidatus ({S}tal) in the transmission of {C}hagas' disease by molecular epidemiology analysis of {T}rypanosoma cruzi isolates from a geographically restricted area in the north of {C}olombia},
  author = {{D}ib, {J}. and {B}arnab{\'e}, {C}hristian and {T}ibayrenc, {M}ichel and {T}riana, {O}.},
  editor = {},
  language = {{ENG}},
  abstract = {{F}ollowing the report of two cases of acute {C}hagas' disease and the appearance of several triatomine species in human dwellings in an area considered non-endemic for domestic transmission of {T}rypanosoma cruzi; a epidemiological, entomological and {T} cruzi molecular epidemiology analysis was performed in order to establish the transmission dynamic of the parasite in the studied area. 2 {T} cruzi isolates from human patients, 5 from {E}ratyrus cuspidatus, 4 from {R}hodnius pallescens, 4 from {P}anstrongylus geniculatus and 7 reference stocks were analyzed by mini-exon gene, random amplified polymorphic {DNA} ({RAPD}) and multilocus enzyme electrophoresis ({MLEE}). {A}ll isolates from vectors and human resulted {T} cruzi group {I} by mini-exon, {RAPD} and {MLEE}. {W}hile mini-exon and {MLEE} did not showed any differences between the studied isolates, {RAPD} analysis identified a common {T} cruzi genotype for the {E}. cuspidatus isolates and human isolates and distinguished different strains from {P}, pallescens and {P}. geniculatus isolates. {T}he presence of the same {T} cruzi genotype in isolates from patients and {E}. cuspidatus suggests that this species can be responsible for the transmission of {C}hagas' disease in the study area. {RAPD} analysis showed better resolution and discrimination of {T} cruzi strains than mini-exon and {MLEE} and can be considered a useful too] for molecular epidemiology studies. {I}ncrimination of sylvatic triatomine species in the transmission of {C}hagas' disease indicates that more knowledge about the ecology of these vectors is necessary to improve control strategies.},
  keywords = {{E}ratyrus cuspidatus ; {T}ryponosoma cruzi ; {M}olecular epidemiology},
  booktitle = {},
  journal = {{A}cta {T}ropica},
  volume = {111},
  numero = {3},
  pages = {237--242},
  ISSN = {0001-706{X}},
  year = {2009},
  DOI = {10.1016/j.actatropica.2009.05.004},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046322},
}