@article{fdi:010046266, title = {{C}urrent genetic differentiation of {C}offea canephora {P}ierre ex {A}. {F}roehn in the {G}uineo-{C}ongolian {A}frican zone : cumulative impact of ancient climatic changes and recent human activities}, author = {{G}omez, {C}{\'e}line and {D}ussert, {S}t{\'e}phane and {H}amon, {P}erla and {H}amon, {S}erge and {K}ochko, {A}lexandre de and {P}oncet, {V}al{\'e}rie}, editor = {}, language = {{ENG}}, abstract = {{B}ackground: {A}mong {C}offea species, {C}. canephora has the widest natural distribution area in tropical {A}frican forests. {I}t represents a good model for analyzing the geographical distribution of diversity in relation to locations proposed as part of the "refuge theory". {I}n this study, we used both microsatellite (simple sequence repeat, {SSR}) and restriction fragment length polymorphism ({RFLP}) markers to investigate the genetic variation pattern of {C}. canephora in the {G}uineo-{C}ongolean distribution zone. {R}esults: {B}oth markers were first compared in terms of their informativeness and efficiency in a study of genetic diversity and relationships among wild {C}. canephora genotypes. {A}s expected, {SSR} markers were found to have a higher genetic distance detection capacity than {RFLP}. {N}evertheless, similarity matrices showed significant correlations when {M}antel's test was carried out (r = 0.66, p < 0.0001). {F}inally, both markers were equally effective for group discrimination and phylogenetic studies, but {SSR} markers tended to outperform {RFLP} markers in discriminating the source of an individual among diversity groups and in putative hybrid detection. {F}ive well defined genetic groups, one in the {U}pper {G}uinean forests, the four others in the {L}ower {G}uinean forests, were identified, corresponding to geographical patterning in the individuals. {C}onclusion: {O}ur data suggested that the {D}ahomey {G}ap, a biogeographical barrier, played a role in wild {C}. canephora differentiation. {C}limatic variations during the {P}leistocene and/or {H}olocene probably caused the subgroup differentiation in the {C}ongolese zone through the presence of a mosaic of putative refugia. {R}ecent hybridization between {C}. canephora diversity groups, both for spontaneous individuals and cultivars, was further characterised according to their geographic dissemination or breeding history as a consequence of human activities.}, keywords = {{AFRIQUE} {SUBSAHARIENNE} ; {ZONE} {TROPICALE}}, booktitle = {}, journal = {{BMC} {E}volutionary {B}iology}, volume = {9}, numero = {}, pages = {167}, ISSN = {1471-2148}, year = {2009}, DOI = {10.1186/1471-2148-9-167}, URL = {https://www.documentation.ird.fr/hor/fdi:010046266}, }