@article{fdi:010046265,
  title = {{I}dentification of total and differentially expressed excreted-secreted proteins from {T}rypanosoma congolense strains exhibiting different virulence and pathogenicity},
  author = {{G}r{\'e}baut, {P}ascal and {C}huchana, {P}aul and {B}rizard, {J}ean-{P}aul and {D}emettre, {E}. and {S}eveno, {M}. and {B}ossard, {G}. and {J}ouin, {P}. and {V}incendeau, {P}. and {B}engaly, {Z}. and {B}oulang{\'e}, {A}. and {C}uny, {G}{\'e}rard and {H}olzmuller, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}nimal trypanosomosis is a major constraint to livestock productivity in the tropics and has a significant impact on the life of millions of people globally (mainly in {A}frica, {S}outh {A}merica and south-east {A}sia). {I}n {A}frica, the disease in livestock is caused mainly by {T}rypanosoma congolense, {T}rypanosoma vivax, {T}rypanosoma evansi and {T}rypanosoma brucei brucei. {T}he extracellular position of trypanosomes in the bloodstream of their host requires consideration of both the parasite and its naturally excreted-secreted factors (secretome) in the course of pathophysiological processes. {W}e therefore developed and standardised a method to produce purified proteomes and secretomes of {A}frican trypanosomes. {I}n this study, two strains of {T} congolense exhibiting opposite properties of both virulence and pathogenicity were further investigated through their secretome expression and its involvement in host-parasite interactions. {W}e used a combined proteomic approach (one-dimensional {SDS}-{PAGE} and two-dimensional differential in-gel electrophoresis coupled to mass spectrometry) to characterise the whole and differentially expressed protein contents of secretomes. {T}he molecular identification of differentially expressed trypanosome molecules and their correlation with either the virulence process or pathogenicity are discussed with regard to their potential as new diagnostic or therapeutic tools against animal trypanosomosis.},
  keywords = {{T}rypanosoma congolense ; {S}ecretome ; {P}roteomics ; {P}athogenic factors},
  booktitle = {},
  journal = {{I}nternational {J}ournal for {P}arasitology},
  volume = {39},
  numero = {10},
  pages = {1137--1150},
  ISSN = {0020-7519},
  year = {2009},
  DOI = {10.1016/j.ijpara.2009.02.018},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046265},
}