@article{fdi:010046241,
  title = {{Q}uantification of key genes steering the microbial nitrogen cycle in the rhizosphere of sorghum cultivars in tropical agroecosystems},
  author = {{H}ai, {B}. and {D}iallo, {N}deye {H}{\'e}l{\`e}ne and {S}all, {S}aidou and {H}aesler, {F}. and {S}chauss, {K}. and {B}onzi, {M}. and {A}ssigbets{\'e}, {K}omi and {C}hotte, {J}ean-{L}uc and {M}unch, {J}. {C}. and {S}chloter, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he effect of agricultural management practices on geochemical cycles in moderate ecosystems is by far better understood than in semiarid regions, where fertilizer availability and climatic conditions are less favorable. {W}e studied the impact of different fertilizer regimens in an agricultural long-term observatory in {B}urkina {F}aso at three different plant development stages ( early leaf development, flowering, and senescence) of sorghum cultivars. {U}sing real-time {PCR}, we investigated functional microbial communities involved in key processes of the nitrogen cycle ( nitrogen fixation, ammonia oxidation, and denitrification) in the rhizosphere. {T}he results indicate that fertilizer treatments and plant development stages combined with environmental factors affected the abundance of the targeted functional genes in the rhizosphere. {W}hile nitrogen-fixing populations dominated the investigated communities when organic fertilizers ( manure and straw) were applied, their numbers were comparatively reduced in urea-treated plots. {I}n contrast, ammonia-oxidizing bacteria ({AOB}) increased not only in absolute numbers but also in relation to the other bacterial groups investigated in the urea-amended plots. {A}mmonia-oxidizing archaea exhibited higher numbers compared to {AOB} independent of fertilizer application. {S}imilarly, denitrifiers were also more abundant in the urea-treated plots. {O}ur data imply as well that, more than in moderate regions, water availability might shape microbial communities in the rhizosphere, since low gene abundance data were obtained for all tested genes at the flowering stage, when water availability was very limited.},
  keywords = {},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {75},
  numero = {15},
  pages = {4993--5000},
  ISSN = {0099-2240},
  year = {2009},
  DOI = {10.1128/aem.02917-08},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046241},
}