Lefèbvre Thomas auteur aut IRD Miambi E. auteur aut IRD Pando Anne auteur aut IRD Diouf Michel auteur aut IRD Rouland Lefèvre Corinne auteur aut IRD text journalArticle eng text/pdf born digital access This comprehensive survey studied the actinobacterial community structure and putative representative members associated with the gut of the wood-feeding termite, Nasutitermes corniger (Motschulsky), using nested PCR-DGGE and 16S rDNA sequences analyses. The closest relatives of the actinobacteria inhabiting the gut of Nasutitermes corniger were in five families, regardless of the geographical origin of the termite colony: Propionibacteriaceae, Streptomycetaceae, Cellulomonodaceae, Corynebacteriaceae and Rubrobacteraceae. Feeding termites on beech wood did not result in substantial changes in the actinobacterial community structure as revealed by DGGE banding patterns. Most of the 16S rDNA sequences obtained after excision and sequencing of DGGE bands clustered with those previously retrieved in termite guts. These results confirm the presence of gut-specific actinobacteria. Except for the 16S rDNA sequences affiliated to Streptomycetaceae and Cellulomonodaceae, no sequence had more than 97% similarity with the closest isolated strains, indicating the presence of microorganisms that have not yet been cultivated. These results suggest that members of the Actinomycetales order account for the largest proportion of the Actinobacteria phylum inhabiting the gut of the termite N. corniger. specialized Actinomycetes Termite gut Nested PCR DGGE 074 56 3 269-276 2009 0020-1812 https://www.documentation.ird.fr/hor/fdi:010046238 10.1007/s00040-009-0020-6 0020-1812 [F B010046238] https://www.documentation.ird.fr/hor/fdi:010046238 https://www.documentation.ird.fr/intranet/publi/2009/08/010046238.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2009-09-01 2017-08-23 fdi:010046238 fre