Tahar Rachida auteur aut IRD Ringwald Pascal auteur aut IRD Basco Leonardo auteur aut IRD text journalArticle eng text/pdf reformatted digital access The Plasmodium falciparum ATPase 6 (Pfatp6), homolog of sarco-endoplasmic reticulum, calcium-dependent ATPase in malaria parasites, has been proposed to be the main target of artemisinins. Four distinct point mutations (L263E, E431K, A623E, and S769N) have been reported to be associated with artemisinin resistance. ne Pfatp6 sequence polymorphism was determined to evaluate the prevalence of these mutations in fresh clinical isolates in Yaounde, Cameroon, and compare sequence data with in vitro response to dihydroartemisinin. Two major haplotypes were observed: the wild-type LEAS (n = 60, 62%) and a single mutant LKAS (n = 35, 36%). These amino acid substitutions did not influence the level of in vitro response to dihydroartemisinin (P > 0.05). Plasmodium falciparum isolates from Cameroon are highly sensitive in vitro to artemisinins. However, the relatively high prevalence of E431K may be a warning signal that warrants a regular monitoring of these molecular markers and/or in vitro activity of artemisinin derivatives. specialized CAMEROUN 052ANOPAL04 050EPID 81 1 13-18 2009 0002-9637 https://www.documentation.ird.fr/hor/fdi:010046224 0002-9637 [F B010046224] https://www.documentation.ird.fr/hor/fdi:010046224 https://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers20-08/010046224.pdf IRD - Base Horizon / Pleins textes 2009-07-24 2023-07-05 fdi:010046224 fre