@article{fdi:010046224,
  title = {{M}olecular epidemiology of malaria in {C}ameroon. {XXVIII}. {I}n vitro activity of dihydroartemisinin against clinical isolates of {P}lasmodium falciparum and sequence analysis of the {P}. falciparum {ATP}ase 6 gene},
  author = {{T}ahar, {R}achida and {R}ingwald, {P}ascal and {B}asco, {L}eonardo},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he {P}lasmodium falciparum {ATP}ase 6 ({P}fatp6), homolog of sarco-endoplasmic reticulum, calcium-dependent {ATP}ase in malaria parasites, has been proposed to be the main target of artemisinins. {F}our distinct point mutations ({L}263{E}, {E}431{K}, {A}623{E}, and {S}769{N}) have been reported to be associated with artemisinin resistance. ne {P}fatp6 sequence polymorphism was determined to evaluate the prevalence of these mutations in fresh clinical isolates in {Y}aounde, {C}ameroon, and compare sequence data with in vitro response to dihydroartemisinin. {T}wo major haplotypes were observed: the wild-type {LEAS} (n = 60, 62%) and a single mutant {LKAS} (n = 35, 36%). {T}hese amino acid substitutions did not influence the level of in vitro response to dihydroartemisinin ({P} > 0.05). {P}lasmodium falciparum isolates from {C}ameroon are highly sensitive in vitro to artemisinins. {H}owever, the relatively high prevalence of {E}431{K} may be a warning signal that warrants a regular monitoring of these molecular markers and/or in vitro activity of artemisinin derivatives.},
  keywords = {{CAMEROUN}},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {81},
  numero = {1},
  pages = {13--18},
  ISSN = {0002-9637},
  year = {2009},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046224},
}