Horizon 1 1 17 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES Experimental Parasitology 353-356 THP-1 Murine macrophages Leishmania Leishmania peruviana Leishmanicidal Amphotericin B 2009 fdi:010046220 ENG Experimental Parasitology 0014-4894 ISI:000267636400014 4 10.1016/j.exppara.2009.05.005 https://www.documentation.ird.fr/hor/fdi:010046220 https://www.documentation.ird.fr/intranet/publi/2009/07/010046220.pdf 122 Horizon (IRD) This study, undertaken to compare the susceptibility of THP-1 cells and murine peritoneal macrophages to Leishmania peruviana amastigotes, obtained THP-1 infection with 10 parasites/cell compared to 2 parasites/murine macrophage. The parasite burden was maximal at 72 h post infection (h.p.i.) for THP-1 cells, while it was still increasing at 120 h.p.i. for murine macrophages. Since in both cases the infection with L peruviana affected cell viability, we recommend evaluating any leishmanicidal activity at 72 h.p.i. Amphotericin B reduced Leishmania infection by 50% at concentrations of 0.1 mu M in THP-1 and murine macrophages at 72 h.p.i. Our results demonstrate that amastigotes of L. peruviana can infect THP-1 cells and murine macrophages and indicate the suitability of this model to screen compounds for leishmanicidal activity. 052 UR152 Pérou