%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Gonzalez, G.
%A Castillo, D.
%A Estevez, Y.
%A Grentzinger, Thomas
%A Deharo, Eric
%T Leishmania (Viannia) peruviana (MHOM/PE/LCA08): Comparison of THP-1 cell and murine macrophage susceptibility to axenic amastigotes for the screening of leishmanicidal compounds
%D 2009
%L fdi:010046220
%G ENG
%J Experimental Parasitology
%@ 0014-4894
%K THP-1 ; Murine macrophages ; Leishmania ; Leishmania peruviana ; Leishmanicidal ; Amphotericin B
%M ISI:000267636400014
%N 4
%P 353-356
%R 10.1016/j.exppara.2009.05.005
%U https://www.documentation.ird.fr/hor/fdi:010046220
%> https://www.documentation.ird.fr/intranet/publi/2009/07/010046220.pdf
%V 122
%W Horizon (IRD)
%X This study, undertaken to compare the susceptibility of THP-1 cells and murine peritoneal macrophages to Leishmania peruviana amastigotes, obtained THP-1 infection with 10 parasites/cell compared to 2 parasites/murine macrophage. The parasite burden was maximal at 72 h post infection (h.p.i.) for THP-1 cells, while it was still increasing at 120 h.p.i. for murine macrophages. Since in both cases the infection with L peruviana affected cell viability, we recommend evaluating any leishmanicidal activity at 72 h.p.i. Amphotericin B reduced Leishmania infection by 50% at concentrations of 0.1 mu M in THP-1 and murine macrophages at 72 h.p.i. Our results demonstrate that amastigotes of L. peruviana can infect THP-1 cells and murine macrophages and indicate the suitability of this model to screen compounds for leishmanicidal activity.
%$ 052