@article{fdi:010046220,
  title = {{L}eishmania ({V}iannia) peruviana ({MHOM}/{PE}/{LCA}08): {C}omparison of {THP}-1 cell and murine macrophage susceptibility to axenic amastigotes for the screening of leishmanicidal compounds},
  author = {{G}onzalez, {G}. and {C}astillo, {D}. and {E}stevez, {Y}. and {G}rentzinger, {T}homas and {D}eharo, {E}ric},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his study, undertaken to compare the susceptibility of {THP}-1 cells and murine peritoneal macrophages to {L}eishmania peruviana amastigotes, obtained {THP}-1 infection with 10 parasites/cell compared to 2 parasites/murine macrophage. {T}he parasite burden was maximal at 72 h post infection (h.p.i.) for {THP}-1 cells, while it was still increasing at 120 h.p.i. for murine macrophages. {S}ince in both cases the infection with {L} peruviana affected cell viability, we recommend evaluating any leishmanicidal activity at 72 h.p.i. {A}mphotericin {B} reduced {L}eishmania infection by 50% at concentrations of 0.1 mu {M} in {THP}-1 and murine macrophages at 72 h.p.i. {O}ur results demonstrate that amastigotes of {L}. peruviana can infect {THP}-1 cells and murine macrophages and indicate the suitability of this model to screen compounds for leishmanicidal activity.},
  keywords = {{THP}-1 ; {M}urine macrophages ; {L}eishmania ; {L}eishmania peruviana ; {L}eishmanicidal ; {A}mphotericin {B}},
  booktitle = {},
  journal = {{E}xperimental {P}arasitology},
  volume = {122},
  numero = {4},
  pages = {353--356},
  ISSN = {0014-4894},
  year = {2009},
  DOI = {10.1016/j.exppara.2009.05.005},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046220},
}