Kumar Roiné Shilpa auteur aut IRD Matsui Mariko auteur aut IRD Reybier K. auteur aut IRD Darius H. T. auteur aut IRD Chinain M. auteur aut IRD Pauillac S. auteur aut IRD Laurent Dominique auteur aut IRD text journalArticle eng text/pdf born digital access Aim of the study: Ciguatera fish poisoning (CFP) is an intertropical ichthyosarcotoxism that manifests in complex assortment of symptoms in humans. Ciguatoxins (CTXs), issued from Gambierdicus spp., are causative agents of this intoxication. We have recently demonstrated that a Pacific CTX (P-CTX-1 B) strongly modulated iNOS expression, leading to overproduction of nitric oxide (NO) in RAW 264.7 murine macrophage cells. NO produced in large amounts is involved in a wide range of pathophysiological processes. Many traditional remedies are commonly used in the Pacific against CFP. In this context, bioassay-guided screening was carried out to study NO inhibiting capacity of 28 selected plant extracts. Materials and methods: We prepared aqueous extracts of plants used in New Caledonia in the treatment of CFP and screened their NO inhibitory activity in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. Results: Among 28 plants tested, Euphorbia hirta (Euphorbiaceae), Syzygium malaccense (Myrtaceae), Schinus terebenthifolius (Anacardiaceae), Punica granatum (Punicaceae), Cerbera manghas (Apocynaceae), Vitex trifolia (Labiateae) and Ximenia americana (Olacaceae) showed inhibitory activity, validating their use as traditional remedies in CFP, and the potential for use in the treatment of conditions accompanied by NO overproduction. Conclusion: These plants are promising candidates for further screening of their active compounds through activity-guided fractionation. specialized Nitric oxide Folk remedies Ciguatera fish poisoning Lipopolysaccharide RAW 264.7 macrophage 076 050 123 3 369-377 2009 0378-8741 https://www.documentation.ird.fr/hor/fdi:010046218 10.1016/j.jep.2009.03.039 0378-8741 [F B010046218] https://www.documentation.ird.fr/hor/fdi:010046218 https://www.documentation.ird.fr/intranet/publi/2009/07/010046218.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2009-07-24 2017-08-23 fdi:010046218 fre