%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Pabon, A.
%A Deharo, Eric
%A Zuluaga, L.
%A Maya, J. D.
%A Saez, J.
%A Blair, S.
%T Plasmodium falciparum : effect of Solanum nudum steroids on thiol contents and beta-hematin formation in parasitized erythrocytes
%D 2009
%L fdi:010046212
%G ENG
%J Experimental Parasitology
%@ 0014-4894
%K Solanum nudum ; Plasmodium falciparum ; Glutathione ; Cysteine ; beta-Hematin
%M ISI:000267636400003
%N 4
%P 273-279
%R 10.1016/j.exppara.2009.04.014
%U https://www.documentation.ird.fr/hor/fdi:010046212
%> https://www.documentation.ird.fr/intranet/publi/2009/07/010046212.pdf
%V 122
%W Horizon (IRD)
%X We studied the effects on total thiols glutathione (GSH) and cysteine contents in Plasmodium falciparum in vitro when treated with four steroid derivatives and a sapogenin (Diosgenone) extracted from Solanum nudum. We also determined their capacity to inhibit beta-hematin formation. We showed that SN-1 (16 alpha-acetoxy-26-hydroxycholest-4-ene-3,22-dione) increased total glutathione and cysteine concentrations while SN-4 (26-O-beta-D-glucopyranosyloxy-16 alpha-acetoxycholest-4-ene-3,22-dione) decreased the concentration of both thiols. Acetylation in C16 was crucial for the effect of SN-1 while type furostanol and terminal glucosidation were necessary for the inhibitory properties of SN-4. The combination of steroids and buthionine sulfoximine, a specific inhibitor of a step-limiting enzyme in GSH synthesis, did not modify the glutathione contents. Finally, we found that SN-1 inhibited more than 80% of beta-hematin formation while the other steroids did not show any effect.
%$ 052