@article{fdi:010046212,
  title = {{P}lasmodium falciparum : effect of {S}olanum nudum steroids on thiol contents and beta-hematin formation in parasitized erythrocytes},
  author = {{P}abon, {A}. and {D}eharo, {E}ric and {Z}uluaga, {L}. and {M}aya, {J}. {D}. and {S}aez, {J}. and {B}lair, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e studied the effects on total thiols glutathione ({GSH}) and cysteine contents in {P}lasmodium falciparum in vitro when treated with four steroid derivatives and a sapogenin ({D}iosgenone) extracted from {S}olanum nudum. {W}e also determined their capacity to inhibit beta-hematin formation. {W}e showed that {SN}-1 (16 alpha-acetoxy-26-hydroxycholest-4-ene-3,22-dione) increased total glutathione and cysteine concentrations while {SN}-4 (26-{O}-beta-{D}-glucopyranosyloxy-16 alpha-acetoxycholest-4-ene-3,22-dione) decreased the concentration of both thiols. {A}cetylation in {C}16 was crucial for the effect of {SN}-1 while type furostanol and terminal glucosidation were necessary for the inhibitory properties of {SN}-4. {T}he combination of steroids and buthionine sulfoximine, a specific inhibitor of a step-limiting enzyme in {GSH} synthesis, did not modify the glutathione contents. {F}inally, we found that {SN}-1 inhibited more than 80% of beta-hematin formation while the other steroids did not show any effect.},
  keywords = {{S}olanum nudum ; {P}lasmodium falciparum ; {G}lutathione ; {C}ysteine ; beta-{H}ematin},
  booktitle = {},
  journal = {{E}xperimental {P}arasitology},
  volume = {122},
  numero = {4},
  pages = {273--279},
  ISSN = {0014-4894},
  year = {2009},
  DOI = {10.1016/j.exppara.2009.04.014},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046212},
}