@article{fdi:010046210,
  title = {{S}pecifc recognition of the major capsid protein of {A}canthamoeba polyphaga mimivirus by sera of patients infected by {F}rancisella tularensis},
  author = {{P}elletier, {N}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{F}rancisella tularensis, a {G}ram-negative cocobacillus responsible for tularemia, especially severe pneumonia, is a facultative intracellular bacterium classified as a biological agent of category {A}. {A}canthamoeba polyphaga mimivirus ({APM}) is a recently discovered giant virus suspected to be an agent of both community-and hospital-acquired pneumonia. {D}uring specificity testing of antibody to {APM} detection, it was observed that nearly all patients infected by {F}. tularensis had elevated antibody titers to {APM}. {I}n the present study, we investigated this cross-reactivity by immunoproteomics. {A}part from the detection of antibodies reactive to new immunoreactive proteins in patients infected by {F}. tularensis, we showed that the sera of those patients recognize specifically two proteins of {APM}: the capsid protein and another protein of unknown function. {N}o common protein motif can be detected in silico based on genome analysis of the involved protein. {F}urthermore, this cross-reactivity was confirmed with the recombinant capsid protein expressed in {E}scherichia coli. {T}his emphasizes the pitfalls of a serological diagnosis of pneumonia.},
  keywords = {tularemia ; cross-reaction ; {M}imivirus ; {W}estern blot ; protein of capsid},
  booktitle = {},
  journal = {{F}ems {M}icrobiology {L}etters},
  volume = {297},
  numero = {1},
  pages = {117--123},
  ISSN = {0378-1097},
  year = {2009},
  DOI = {10.1111/j.1574-6968.2009.01675.x},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046210},
}