@article{fdi:010046200,
  title = {{P}urification and assay of {A}rapaima gigas vitellogenin : potential use for sex determination},
  author = {{D}ugu{\'e}, {R}{\'e}mi and {K}oo, {F}. {C}. and {A}lcantara, {F}. and {D}uponchelle, {F}abrice and {R}enno, {J}ean-{F}rancois and {N}unez, {J}esus},
  editor = {},
  language = {{ENG}},
  abstract = {{A}rapaima gigas is an ancient, air-breathing, giant fish of {A}mazonian rivers. {D}ue to its economic importance, the development of aquaculture for this species is currently a good alternative to counteract the decline of wild populations. {R}eproduction in captivity relies on the constitution of male and female pairs, which occur naturally in large ponds. {B}ut, as sex determination of {A}. gigas is not possible by morphological criteria it is very difficult to optimize reproduction success and fry production in each pond. {T}his situation prompted us to develop a sexing methodology based on the detection of female specific plasma {V}itellogenin ({V}tg) using an {E}nzyme {I}mmuno-{A}ssay ({EIA}). {V}itellogenin purification was performed by electro-elution after polyacrilamide gel electrophoresis ({PAGE}) from plasma of 17 beta-{E}stradiol treated {A}. gigas juveniles. {T}wo different {V}tg molecules were isolated, ({V}t(g1) and {V}t(g2)) with 184 and 112 k{D}a apparent molecular masses repectively. {B}oth antibodies, produced in rabbits, reacted with both vitellogenins in a competitive assay or by direct {V}tg coating. {F}or sexing of the fish, we finally chose the method of direct coating of serial plasma dilutions.},
  keywords = {{A}rapaima gigas ; {S}ex determination ; {V}itellogenin, {EIA} ; {A}mazon},
  booktitle = {},
  journal = {{C}ybium},
  volume = {32},
  numero = {2 suppl.},
  pages = {111--111},
  ISSN = {0399-0974},
  year = {2008},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046200},
}