@article{fdi:010046184,
  title = {{P}yrosequencing of tagged 16{S} r{RNA} gene amplicons for rapid deciphering of the microbiomes of fermented foods such as pearl millet slurries},
  author = {{H}umblot, {C}hrist{\`e}le and {G}uyot, {J}ean-{P}ierre},
  editor = {},
  language = {{ENG}},
  abstract = {{P}earl millet slurries, mixed with groundnuts or not, were chosen as a model to investigate the feasibility of obtaining a rapid overview of community structure and population dynamics of fermented foods using pyro-sequencing of tagged 16{S} r{RNA} gene amplicons. {F}rom 14 fermented samples collected either in a traditional small-scale processing unit in {B}urkina {F}aso or at laboratory scale, 137,469 sequences of bacterial 16{S} r{RNA} gene amplicons were characterized. {E}xcept for a few {P}roteobacteria, almost all the bacterial sequences were attributed to cultivable bacteria. {T}his approach enabled 80.7% of the sequences to be attributed to a family and 70% to a genus but did not enable identification to the species level. {T}he bacterial sequences were assigned to four phyla, with {F}irmicutes representing the highest diversity, followed by {P}roteobacteria, {A}ctinobacteria, and {B}acteroidetes, which were found only in the slurries prepared in traditional production units. {M}ost of the {F}irmicutes were lactic acid bacteria, mainly represented by members of the {L}actobacillus, {P}ediococcus, {L}euconostoc, and {W}eissella genera, whose ratio varied from the onset to the end of the fermentation. {T}he other bacteria present at the beginning of fermentation were generally no longer detected at the end, which is consistent with already-known patterns in the microbial ecology of fermented foods. {I}n conclusion, this method seems very promising for rapid and preliminary microbial characterization in many samples of an unknown food sample, by determining numerous nucleic sequences simultaneously without the need for cloning and cultivation-dependent methods.},
  keywords = {},
  booktitle = {},
  journal = {{A}pplied and {E}nvironmental {M}icrobiology},
  volume = {75},
  numero = {13},
  pages = {4354--4361},
  ISSN = {0099-2240},
  year = {2009},
  DOI = {10.1128/aem.00451-09},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046184},
}