@article{fdi:010046156,
  title = {{D}evelopment and validation of real-time one-step reverse transcription-{PCR} for the detection and typing of dengue viruses},
  author = {{L}eparc {G}offart, {I}. and {B}aragatti, {M}. and {T}emmam, {S}. and {T}uiskunen, {A}. and {M}oureau, {G}. and {C}harrel, {R}. and de {L}amballerie, {X}avier},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {D}engue virus, transmitted by mosquitoes, causes every year 50 million cases of dengue fever. {A} standardize method for early diagnosis is still needed for clinical diagnosis and epidemiological {S}tudies. {O}bjective: {T}o develop and validate for sensitivity, specificity, linearity and precision real-time one-step {RT}-{PCR} for the detection of dengue viruses. {S}tudy design: {M}ultiple alignments of dengue virus sequence for each serotype were done and used to develop five systems of real-time {RT}-{PCR} to detect all dengue virus strains and then identify the serotype. {T}hese systems were validated on synthetic {RNA} transcripts for specificity, sensitivity, precision and linearity and then applied on series of human samples. {R}esults:{T}he specificity of each system was determined by sequence alignments and experimentally tested on different flaviviruses. {M}ethods precision and linearity were statistically validated. {E}ach of these systems allowed the detection of less than one infectious particle and was able to detect and serotype quickly dengue virus in human samples where infectious virus cannot be isolated anymore. {C}onclusions: {T}hese systems are valuable tools for dengue virus diagnosis and epidemiological {S}tudies. {S}tandardization and validation of these methods allow an easy transfer to diagnostic laboratories.},
  keywords = {{D}engue virus ; {D}iagnosis ; {R}eal-time {RT}-{PCR} ; {V}alidation ; {S}tandardization},
  booktitle = {},
  journal = {{J}ournal of {C}linical {V}irology},
  volume = {45},
  numero = {1},
  pages = {61--66},
  ISSN = {1386-6532},
  year = {2009},
  DOI = {10.1016/j.jcv.2009.02.010},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046156},
}