@article{fdi:010046148,
  title = {{R}evised {M}imivirus major capsid protein sequence reveals intron-containing gene structure and extra domain},
  author = {{A}zza, {S}. and {C}ambillau, {C}. and {R}aoult, {D}idier and {S}uzan-{M}onti, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {A}canthamoebae polyphaga {M}imivirus ({APM}) is the largest known ds{DNA} virus. {T}he viral particle has a nearly icosahedral structure with an internal capsid shell surrounded with a dense layer of fibrils. {A} {C}apsid protein sequence, {D}13{L}, was deduced from the {APM} {L}425 coding gene and was shown to be the most abundant protein found within the viral particle. {H}owever this protein remained poorly characterised until now. {A} revised protein sequence deposited in a database suggested an additional {N}-terminal stretch of 142 amino acids missing from the original deduced sequence. {T}his result led us to investigate the {L}425 gene structure and the biochemical properties of the complete {APM} major {C}apsid protein. {R}esults: {T}his study describes the full length 3430 bp {C}apsid coding gene and characterises the 593 amino acids long corresponding {C}apsid protein 1. {T}he recombinant full length protein allowed the production of a specific monoclonal antibody able to detect the {C}apsid protein 1 within the viral particle. {T}his protein appeared to be post-translationnally modified by glycosylation and phosphorylation. {W}e proposed a secondary structure prediction of {APM} {C}apsid protein 1 compared to the {C}apsid protein structure of {P}aramecium {B}ursaria {C}hlorella {V}irus 1, another member of the {N}ucleo-{C}ytoplasmic {L}arge {DNA} virus family. {C}onclusion: {T}he characterisation of the full length {L}425 {C}apsid coding gene of {A}canthamoebae polyphaga {M}imivirus provides new insights into the structure of the main {C}apsid protein. {T}he production of a full length recombinant protein will be useful for further structural studies.},
  keywords = {},
  booktitle = {},
  journal = {{B}mc {M}olecular {B}iology},
  volume = {10},
  numero = {},
  pages = {39},
  ISSN = {1471-2199},
  year = {2009},
  DOI = {10.1186/1471-2199-10-39},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046148},
}