@article{fdi:010046134,
  title = {{ASE} extraction method for simultaneous carbon and nitrogen stable isotope analysis in soft tissues of aquatic organisms},
  author = {{B}odin, {N}athalie and {B}udzinski, {H}. and {L}e {M}enach, {K}. and {T}apie, {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}ince lipids are depleted in {C}-13 relative to proteins and carbohydrates, variations in lipid composition among species and within individuals significantly influence delta {C}-13 and may result in misleading ecological interpretations. {W}hereas lipid extraction before {IRMS} analysis constitutes a way of stable isotope result lipid-normalisation, such a procedure was given up because of the un-controlled effects of the methods used (i.e., "{B}ligh & {D}yer", {S}oxhlet, etc.) on delta {N}-15. {T}he aim of this work was to develop a simple, rapid and efficient lipid extraction method allowing for simultaneous {C} and {N} stable isotope analysis in the biological soft tissues of aquatic {O}rganisms. {T}he goal was to be free from the lipid influence on delta {C}-13 values without interfering with delta {N}-15 values. {F}or that purpose, the modern automated pressurized liquid extraction technique {ASE} (accelerated solvent extraction) was selected. {E}el muscles representative of a broad range of far contents were extracted via {ASE} by using different semi-polar solvents (100% dichloromethane and 80% n-hexane/20% acetone) and by operating at different temperature (ambient temperature and 100 degrees {C}) and pressure (750 and 1900 psi) conditions. {T}he results were discussed in terms of lipid extraction efficiency as well as delta {C}-13 and delta {N}-15 variability.},
  keywords = {delta {N}-15 and delta {C}-13 ; {A}ccelerated solvent extraction ; {F}at content ; {M}uscle ; {F}ish},
  booktitle = {},
  journal = {{A}nalytica {C}himica {A}cta},
  volume = {643},
  numero = {1-2},
  pages = {54--60},
  ISSN = {0003-2670},
  year = {2009},
  DOI = {10.1016/j.aca.2009.03.048},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046134},
}