%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Jaubert, Marianne
%A Hannibal, Laure
%A Fardoux, Joël
%A Giraud, Eric
%A Verméglio, A.
%T Identification of novel genes putatively involved in the photosystem synthesis of Bradyrhizobium sp ORS 278
%D 2009
%L fdi:010046131
%G ENG
%J Photosynthesis Research
%@ 0166-8595
%K Photosystem regulation ; Aerobic photosynthetic bacteria ; Bradyrhizobium
%M ISI:000266831200005
%N 2
%P 97-105
%R 10.1007/s11120-009-9433-3
%U https://www.documentation.ird.fr/hor/fdi:010046131
%> https://www.documentation.ird.fr/intranet/publi/2009/06/010046131.pdf
%V 100
%W Horizon (IRD)
%X In aerobic anoxygenic phototrophs, oxygen is required for both the formation of the photosynthetic apparatus and an efficient cyclic electron transfer. Mutants of Bradyrhizobium sp. ORS278 affected in photosystem synthesis were selected by a bacteriochlorophyll fluorescence-based screening. Out of the 9,600 mutants of a random Tn5 insertion library, 50 clones, corresponding to insertions in 28 different genes, present a difference in fluorescence intensity compared to the WT. Besides enzymes and regulators known to be involved in photosystem synthesis, 14 novel components of the photosynthesis control are identified. Among them, two genes, hsIU and hsIV, encode components of a protein degradation complex, probably linked to the renewal of photosystem, an important issue in Bradyrhizobia which have to deal with harmful reactive oxygen species. The presence of homologs in non-photosynthetic bacteria for most of the regulatory genes identified during study suggests that they could be global regulators, as the RegA-RegB system.
%$ 084