@article{fdi:010046115, title = {{E}valuation of the {L}ight{C}ycler{A} ({R}) {S}epti{F}ast test in the rapid etiologic diagnostic of infectious endocarditis}, author = {{C}asalta, {J}. {P}. and {G}ouriet, {F}. and {R}oux, {V}. and {T}huny, {F}. and {H}abib, {G}. and {R}aoult, {D}idier}, editor = {}, language = {{ENG}}, abstract = {{T}he {S}epti{F}ast test ({R}oche {D}iagnostics) is a new commercial molecular technique that has emerged for the detection of bacteria in blood. {W}e compared in this study the sensitivity of blood culture to a commercially available broad-range real-time polymerase chain reaction ({PCR}) assay for the detection in blood of 19 bacterial species and six fungal species ({S}epti{F}ast test, {R}oche {D}iagnostics) in 63 patients with infectious endocarditis ({IE}). {T}he {S}epti{F}ast test is not more sensitive for organisms such as {S}treptococci, {E}nterococci, and {S}taphylococcus aureus (11/29 versus 12/29 for blood culture). {I}t has detected less commonly coagulase-negative {S}taphylococci (0/15 versus 3/15, {P} = 0.2) and significantly fewer other microorganisms (0/6 versus 4/6, {P} = 0.03). {H}owever, bacteria were detected from three {IE} treated by antibiotics, with blood culture negative on admission. {T}he {S}epti{F}ast test may be useful in cases of {IE} in patients treated with antibiotics before admission.}, keywords = {}, booktitle = {}, journal = {{E}uropean {J}ournal of {C}linical {M}icrobiology and {I}nfectious {D}iseases}, volume = {28}, numero = {6}, pages = {569--573}, ISSN = {0934-9723}, year = {2009}, DOI = {10.1007/s10096-008-0672-6}, URL = {https://www.documentation.ird.fr/hor/fdi:010046115}, }