Ribaut C. auteur aut IRD Reybier K. auteur aut IRD Reynes O. auteur aut IRD Launay J. auteur aut IRD Valentin A. auteur aut IRD Fabre P. L. auteur aut IRD Nepveu Françoise auteur aut IRD text journalArticle eng text/pdf born digital access The malaria parasite, Plasmodium falciparum, invades human erythrocytes and induces dramatic changes in the host cell. The idea of this work was to use RBC modified electrode to perform electrochemical impedance spectroscopy (EIS) with the aim of monitoring physiological changes affecting the erythrocyte after invasion by the malaria parasite. Impedance cell-based devices are potentially useful to give insight into cellular behavior and to detect morphological changes. The modelling of impedance plots (Nyquist diagram) in equivalent circuit taking into account the presence of the cellular layer, allowed us pointing out specific events associated with the development of the parasite such as (i) strong changes in the host cell cytoplasm illustrated by changes in the film capacity, (ii) perturbation of the ionic composition of the host cell illustrated by changes in the film resistance, (iii) releasing of reducer (lactic acid or heme) and an enhanced oxygen consumption characterized by changes in the charge transfer resistance and in the Warburg coefficient characteristic of the redox species diffusion. These results show that the RBC-based device may help to analyze strategic events in the malaria parasite development constituting a new tool in antimalarial research. specialized Plasmodium falciparum Red blood cell Electrochemical impedance spectroscopy 052 24 8 2721-2725 2009 0956-5663 https://www.documentation.ird.fr/hor/fdi:010046052 10.1016/j.bios.2008.12.018 0956-5663 [F B010046052] https://www.documentation.ird.fr/hor/fdi:010046052 https://www.documentation.ird.fr/intranet/publi/2009/05/010046052.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2009-06-08 2017-08-23 fdi:010046052 fre