@article{fdi:010046015,
  title = {{T}he expansion of the microbiological spectrum of brain abscesses with use of multiple 16{S} ribosomal {DNA} sequencing},
  author = {{A}l {M}asalma, {M}. and {A}rmougom, {F}. and {S}cheld, {W}. {M}. and {D}ufour, {H}. and {R}oche, {P}. {H}. and {D}rancourt, {M}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground. {B}rain abscess is commonly treated using empirically prescribed antibiotics. {T}hus, a comprehensive study of bacterial organisms associated with brain abscess is essential to define the best empirical treatment for this life-threatening condition. {M}ethods. {W}e prospectively compared cultures to single and multiple sequenced 16{S} ribosomal {DNA} polymerase chain reaction amplifications (by cloning and/or pyrosequencing) of cerebral abscesses in 20 patients from 2 hospitals in {M}arseilles, {F}rance, during the period {J}anuary 2005 through {D}ecember 2007. {R}esults. {T}he obtained cultures identified significantly fewer types of bacteria (22 strains) than did molecular testing (72 strains; {P} = .017, by analysis of variance test). {W}e found that a patient could exhibit as many as 16 different bacterial species in a single abscess. {T}he obtained cultures identified 14 different species already known to cause cerebral abscess. {S}ingle sequencing performed poorly, whereas multiple sequencing identified 49 species, of which 27 had not been previously reported in brain abscess investigations and 15 were completely unknown. {I}nterestingly, we observed 2 patients who harbored {M}ycoplasma hominis (an emerging pathogen in this situation) and 3 patients who harbored {M}ycoplasma faucium, which, to our knowledge, has never been reported in literature. {C}onclusions. {M}olecular techniques dramatically increased the number of identified agents in cerebral abscesses. {M}ycoplasma species are common and should be detected in this situation. {T}hese findings led us to question the accuracy of the current empirical treatment of brain abscess.},
  keywords = {},
  booktitle = {},
  journal = {{C}linical {I}nfectious {D}iseases},
  volume = {48},
  numero = {9},
  pages = {1169--1178},
  ISSN = {1058-4838},
  year = {2009},
  DOI = {10.1086/597578},
  URL = {https://www.documentation.ird.fr/hor/fdi:010046015},
}