Biass D. auteur aut IRD Dutertre S. auteur aut IRD Gerbault A. auteur aut IRD Menou Jean-Louis auteur aut IRD Offord R. auteur aut IRD Favreau P. auteur aut IRD Stocklin R. auteur aut IRD text journalArticle eng text/pdf born digital access In the context of an exhaustive study of the piscivorous cone snail Conus consors, we performed an in-depth analysis of the intact molecular masses that can be detected in the animal's venom, using MALDI and ESI mass spectrometry. We clearly demonstrated that, for the venom of this species at least, it is essential to use both techniques in order to obtain the broadest data set of molecular masses. Only 20% of the total number of molecules detected were found in both mass lists. The two data sets were also compared in terms of mass range and relative hydrophobicity of the components detected in each. With a view to an extensive analysis of this venom's proteome, we further performed a comparative study by ESI-MS between venom obtained after classical dissection of the venom duct versus venom obtained by milking live animals. Surprisingly, although many fewer components were found in the milked venom than in the dissected venom, similar to 50% of those found had not been seen in the dissected venom. Several questions raised by these observations are discussed. With regards to the current knowledge of the cone snail venom composition, our results emphasize the complementary nature of the mass spectrometry methods and of the two techniques used in venom collection. specialized Cone snail Conopeptide Conotoxin Conus consors ESI-MS HPLC Hydrophobicity MALDI-TOF-MS Mass fingerprint Mass range Mass spectrometry Peptide Peptidomics Proteomics Toxins Venom Venomics 034 020 72 2 210-218 2009 1874-3919 https://www.documentation.ird.fr/hor/fdi:010044358 10.1016/j.jprot.2009.01.019 1874-3919 [F B010044358] https://www.documentation.ird.fr/hor/fdi:010044358 https://www.documentation.ird.fr/intranet/publi/2009/04/010044358.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2009-05-13 2017-08-23 fdi:010044358 fre