@article{fdi:010044347,
  title = {{G}lobal proteomic pattern of {T}ropheryma whipplei : a {W}hipple's disease bacterium},
  author = {{K}owalczewska, {M}. and {V}illard, {C}. and {L}afitte, {D}. and {F}enollar, {F}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he proteome of {T}ropheryma whipplei, the intracellular bacterium responsible for {W}hipple's disease ({WD}), was analyzed using two complementary approaches: 2-{DE} coupled with {MALDI}-{TOF} and {SDS}-{PAGE} with nano{LC}-{MS}/{MS}. {T}his strategy led to the identification of 206 proteins of 808 predicted {ORF}s, resolving some questions raised by the genomic sequence of this bacterium. {W}e successfully identified antibiotic targets and proteins with predicted {N}-terminal signal sequences. {A}dditionally, we identified a family of surface proteins (known as {T} whipplei surface proteins ({W}i{SP}s)), which are encoded by a unique group of species-specific genes and serve as both coding regions and {DNA} repeats that promote genomic recombination. {C}omparison of the protein expression profiles of the intracellular facultative host-associated {WD} bacterium with other host-associated, intracellular obligate, and environmental bacteria revealed that {T} whipplei shares a proteomic expression profile with other host-associated facultative intracellular bacteria. in summary, this study describes the global protein expression pattern of {T} whipplei and reveals some specific features of the {T} whipplei proteome.},
  keywords = {{G}enome sequence ; {P}aralogous genes ; {P}rotein profile ; {T}ropheryma whipplei ; {W}i{SP}s family proteins},
  booktitle = {},
  journal = {{P}roteomics},
  volume = {9},
  numero = {6},
  pages = {1593--1616},
  ISSN = {1615-9853},
  year = {2009},
  DOI = {10.1002/pmic.200700889},
  URL = {https://www.documentation.ird.fr/hor/fdi:010044347},
}