@article{fdi:010044336,
  title = {{M}icrocollinearity in an ethylene receptor coding gene region of the {C}offea canephora genome is extensively conserved with {V}itis vinifera and other distant dicotyledonous sequenced genomes},
  author = {{G}uyot, {R}omain and {D}e la {M}are, {M}arion and {V}iader, {V}{\'e}ronique and {H}amon, {P}erla and {C}oriton, {O}. and {B}ustamante {P}orras, {J}os{\'e} and {P}oncet, {V}al{\'e}rie and {C}ampa, {C}laudine and {H}amon, {S}erge and {K}ochko, {A}lexandre de},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {C}offea canephora, also called {R}obusta, belongs to the {R}ubiaceae, the fourth largest angiosperm family. {T}his diploid species (2x = 2n = 22) has a fairly small genome size of approximate to 690 {M}b and despite its extreme economic importance, particularly for developing countries, knowledge on the genome composition, structure and evolution remain very limited. {H}ere, we report the 160 kb of the first {C}. canephora {B}acterial {A}rtificial {C}hromosome ({BAC}) clone ever sequenced and its fine analysis. {R}esults: {T}his clone contains the {C}c{EIN}4 gene, encoding an ethylene receptor, and twenty other predicted genes showing a high gene density of one gene per 7.8 kb. {M}ost of them display perfect matches with {C}. canephora expressed sequence tags or show transcriptional activities through {PCR} amplifications on c{DNA} libraries. {T}wenty-three transposable elements, mainly {C}lass {II} transposon derivatives, were identified at this locus. {M}ost of these {C}lass {II} elements are {M}iniature {I}nverted-repeat {T}ransposable {E}lements ({MITE}) known to be closely associated with plant genes. {T}his {BAC} composition gives a pattern similar to those found in gene rich regions of {S}olanum lycopersicum and {M}edicago truncatula genomes indicating that the {C}c{EIN}4 regions may belong to a gene rich region in the {C}. canephora genome. {C}omparative sequence analysis indicated an extensive conservation between {C}. canephora and most of the reference dicotyledonous genomes studied in this work, such as tomato ({S}. lycopersicum), grapevine ({V}. vinifera), barrel medic {M}. truncatula, black cottonwood ({P}opulus trichocarpa) and {A}rabidopsis thaliana. {T}he higher degree of microcollinearity was found between {C}. canephora and {V}. vinifera, which belong respectively to the {A}sterids and {R}osids, two clades that diverged more than 114 million years ago. {C}onclusion: {T}his study provides a first glimpse of {C}. canephora genome composition and evolution. {O}ur data revealed a remarkable conservation of the microcollinearity between {C}. canephora and {V}. vinifera and a high conservation with other distant dicotyledonous reference genomes. {A}ltogether, these results provide valuable information to identify candidate genes in {C}. canephora genome and serve as a foundation to establish strategies for whole genome sequencing. {F}uture large-scale sequence comparison between {C}. canephora and reference sequenced genomes will help in understanding the evolutionary history of dicotyledonous plants.},
  keywords = {},
  booktitle = {},
  journal = {{B}mc {P}lant {B}iology},
  volume = {9},
  numero = {},
  pages = {22},
  ISSN = {1471-2229},
  year = {2009},
  DOI = {10.1186/1471-2229-9-22},
  URL = {https://www.documentation.ird.fr/hor/fdi:010044336},
}