Hedi A. auteur aut IRD Fardeau Marie-Laure auteur aut IRD Sadfi N. auteur aut IRD Boudabous A. auteur aut IRD Ollivier Bernard auteur aut IRD Cayol Jean-Luc auteur aut IRD text journalArticle eng text/pdf born digital access A new halophilic anaerobe was isolated from the hypersaline surface sediments of El-Djerid Chott, Tunisia. The isolate, designated as strain 6SANG, grew at NaCl concentrations ranging from 14 to 30%, with an optimum at 20-22%. Strain 6SANG was a non-spore-forming, non-motile, rod-shaped bacterium, appearing singly, in pairs, or occasionally as long chains (0.7-1 x 4-13 mu m) and showed a Gram-negative-like cell wall pattern. It grew optimally at pH values between 7.2 and 7.4, but had a very broad pH range for growth (5.9-8.4). Optimum temperature for growth was 42A degrees C (range 30-50A degrees C). Strain 6SANG required yeast extract for growth on sugars. Glucose, sucrose, galactose, mannose, maltose, cellobiose, pyruvate, and starch were fermented. The end products from glucose fermentation were acetate, butyrate, lactate, H-2, and CO2. The G + C ratio of the DNA was 34.3 mol%. Strain 6SANG exhibited 16S rRNA gene sequence similarity values of 91-92% with members of the genus Halobacteroides, H. halobius being its closest phylogenetic relative. Based on phenotypic and phylogenetic characteristics, we propose that this bacterium be classified as a novel species of a novel genus, Halanaerobaculum tunisiense gen. nov., sp. nov. The type strain is 6SANG(T) (=DSM 19997(T) = JCM 15060(T)). specialized Halanaerobaculum tunisiense Halanaerobiales Anaerobic Extreme halophilic 084 13 2 313-319 2009 1431-0651 https://www.documentation.ird.fr/hor/fdi:010044300 10.1007/s00792-008-0218-y 1431-0651 [F B010044300] https://www.documentation.ird.fr/hor/fdi:010044300 https://www.documentation.ird.fr/intranet/publi/2009/03/010044300.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2009-04-08 2020-07-08 fdi:010044300 fre