Adekambi T. auteur aut IRD Drancourt M. auteur aut IRD Raoult Didier auteur aut IRD text journalArticle eng text/pdf born digital access The rpoB gene, encoding the beta-subunit of RNA polymerase, has emerged as a core gene candidate for phylogenetic analyses and identification of bacteria, especially when studying closely related isolates. Together with the 16S rRNA gene, rpoB has helped to delineate new bacterial species and refine bacterial community analysis, as well as enabling the monitoring of rifampicin resistance-conferring mutations. Sequencing of rpoB enables efficient estimation of bacterial G+C% content, DNA-DNA hybridization value and average nucleotide identity (percentage of the total genomic sequence shared between two strains) when taxonomic relationships have been firmly established. New identification tools targeting a rpoB gene fragment located between positions 2300 and 3300 have been developed recently. Therefore, inclusion of the rpoB gene sequence would be useful when describing new bacterial species. specialized 050 17 1 37-45 2009 0966-842X https://www.documentation.ird.fr/hor/fdi:010044256 10.1016/j.tim.2008.09.008 0966-842X [F B010044256] https://www.documentation.ird.fr/hor/fdi:010044256 https://www.documentation.ird.fr/intranet/publi/2009/02/010044256.pdf Accès réservé (Intranet de l'IRD) IRD - Base Horizon / Pleins textes 2009-03-06 2017-08-23 fdi:010044256 fre